Its rattle is so small it can be heard only a few feet away and is easily mistaken for the buzz of an insect. In undisturbed populations, this species may occur in considerable numbers on account of their specialized habitat preference and the abundance of food; the primary prey species being Yarrow's spiny and bunch-grass lizards.

Twin-spotted rattlesnakes pursue active patterns of thermoregulation within the broad range of temperatures in their montane environment. By basking in the sun for short periods, they are able to raise their body temperature above that of their surroundings, an advantage for feeding and other life functions. However, most of their time is spent at moderate or even low temperatures while in their rocky refuge.